Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FBXO8 / FBX8 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G114591-100
Catalog Number: LS-G114591
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FBXO8 (NM_012180), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DC10 | F-box protein Fbx8 | FBX8 | F-box only protein 8 | F-box protein 8 | FBS | FBXO8 | F-box/SEC7 protein FBS
- Origin Species
- Human
- NCBI GenBank
- NM_012180
- SwissProt
- Q9NRD0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 36.9 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MGQGLWRVVR NQQLQQEGYS EQGYLTREQS RRMAASNISN TNHRKQVQGG IDIYHLLKAR61KSKEQEGFIN LEMLPPELSF TILSYLNATD LCLASCVWQD LANDELLWQG LCKSTWGHCS121IYNKNPPLGF SFRKLYMQLD EGSLTFNANP DEGVNYFMSK GILDDSPKEI AKFIFCTRTL181NWKKLRIYLD ERRDVLDDLV TLHNFRNQFL PNALREFFRH IHAPEERGEY LETLITKFSH241RFCACNPDLM RELGLSPDAV YVLCYSLILL SIDLTSPHVK NKMSKREFIR NTRRAAQNIS301EDFVGHLYDN IYLIGHVAATRTRPLEQKLISEEDLAANDILDYKDDDDKV
319 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.