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FBXO46 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FBXO46 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G119342-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FBXO46 (NM_001080469), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
20D7-FC4 | FBXO34L | F-box only protein 46 | F-box protein 46 | Fbx46 | FBXO46 | F-box only protein 34-like
Origin Species
Human
NCBI GenBank
NM_001080469
SwissProt
Q6PJ61

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
64.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDRGSLLPFQ LWCPRPFGTY SQNQPRPPSA ALKPSACPEP GGGAEPDHGP AHSENTPPAL
61ATEVPASQPA PLLSAAAAGD EGRVLLDTWY VIKPGNTKEK VAFFVAHQCG GGSRASSMKV
121KGHWGSDSSK AKRRRRCLDP TKAPPDPGGR EGPPAAEEGP ASAGEDVDLL SVAEMVALVE
181QRAALALQSY PRPTTPAPVV FVSAEQGGPA KGVGSERRSG GGDCSRVAEA VAHFEAQRDS
241PPTKGLRKEE RPGPGPGEVR IAFRISNGRE PRAPDSGLPS GGGGRPGCAY PGSPGPGARA
301KDKITCDLYQ LISPSRDALP SNVEFLLARA DEASEGDSPA PARPEDTPPA PPPPPARDCG
361ASGFHVDVVV TGVVDECIFF GKDGTKNVKE ETVCLTVSPE EPPPPGQLFF LQNRGPDGPP
421EPPPADSPAT APGPDDAEGT ADTSLCRLYR HVSHDFLEIR FKIQRLLEPR QYMLLLPEHV
481LVKIFSFLPT RALAALKCTC HHFKGIIEAF GVRATDSRWS RDPLYRDDPC KQCRKRYEKG
541DVSLCRWHPK PYHHDLPYGR SYWMCCRRAD RETPGCRLGL HDNNWVLPCN GPGGGRAGRE
601EGR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

603 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.