Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FBXO42 / JFK Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G111512-100
Catalog Number: LS-G111512
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FBXO42 (NM_018994), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- F-box only protein 42 | F-box protein 42 | Fbx42 | FBXO42 | KIAA1332 | JFK
- Origin Species
- Human
- NCBI GenBank
- H79973
- SwissProt
- Q6P3S6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 77.7 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MASSSDSEDD SFMAVDQEET VLEGTMDQDE EPHPVLEAEE TRHNRSMSEL PEEVLEYILS61FLSPYQEHKT AALVCKQWYR LIKGVAHQCY HGFMKAVQEG NIQWESRTYP YPGTPITQRF121SHSACYYDAN QSMYVFGGCT QSSCNAAFND LWRLDLNSKE WIRPLASGSY PSPKAGATLV181VYKDLLVLFG GWTRPSPYPL HQPERFFDEI HTYSPSKNWW NCIVTTHGPP PMAGHSSCVI241DDKMIVFGGS LGSRQMSNDV WVLDLEQWAW SKPNISGPSP HPRGGQSQIV IDDATILILG301GCGGPNALFK DAWLLHMHSG PWAWQPLKVE NEEHGAPELW CHPACRVGQC VVVFSQAPSG361RAPLSPSLNS RPSPISATPP ALVPETREYR SQSPVRSMDE APCVNGRWGT LRPRAQRQTP421SGSREGSLSP ARGDGSPILN GGSLSPGTAA VGGSSLDSPV QAISPSTPSA PEGYDLKIGL481SLAPRRGSLP DQKDLRLGSI DLNWDLKPAS SSNPMDGMDN RTVGGSMRHP PEQTNGVHTP541PHVASALAGA VSPGALRRSL EAIKAMSSKG PSASAALSPP LGSSPGSPGS QSLSSGETVP601IPRPGPAQGD GHSLPPIARR LGHHPPQSLN VGKPLYQSMN CKPMQMYVLD IKDTKEKGRV661KWKVFNSSSV VGPPETSLHT VVQGRGELII FGGLMDKKQN VKYYPKTNAL YFVRAKRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
717 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.