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FBXO40 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FBXO40 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FBXO40 antibodies →
$725.00each
Catalog Number: LS-G112742-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FBXO40 (NM_016298), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
F-box only protein 40 | F-box protein 40 | FBX40 | FBXO40 | KIAA1195 | Muscle disease-related protein
Origin Species
Human
NCBI GenBank
NM_016298
SwissProt
Q9UH90

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
79.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGKARRSPPG HHRHCEGCFN RHCHIPVEPN TSCLVISCHL LCGATFHMCK EAEHQLLCPL
61EQVPCLNSEY GCPLSMSRHK LAKHLQVCPA SVVCCSMEWN RWPNVDSETT LHENIMKETP
121SEECLDTALA LQDQKVLFRS LKMVELFPET REATEEEPTM NGETSVEEMG GAVGGVDIGL
181VPHGLSATNG EMAELSQEER EVLAKTKEGM DLVKFGQWEN IFSKEHAASA LTNSSASCES
241KNKNDSEKEQ ISSGHNMVEG EGAPKKKEPQ ENQKQQDVRT AMETTGLAPW QDGVLERLKT
301AVDAKDYNMY LVHNGRMLIH FGQMPACTPK ERDFVYGKLE AQEVKTVYTF KVPVSYCGKR
361ARLGDAMLSC KPSEHKAVDT SDLGITVEDL PKSDLIKTTL QCALERELKG HVISESRSID
421GLFMDFATQT YNFEPEQFSS GTVLADLTAA TPGGLHVELH SECVTRRHNK SSSAFTFTCN
481KFFRRDEFPL HFKNVHTDIQ SCLNGWFQHR CPLAYLGCTF VQNHFRPPGQ KAKVIYSQEL
541KTFAIKPEVA PELSEGRKNN HLLGHGGKSQ NSLTSLPLEI LKYIAGFLDS VSLAQLSQVS
601VLMRNICATL LQERGMVLLQ WKKKRYSHGG TSWRVHREIW QFSSLFSKIK SWEFNEVTSM
661SEHLKSCPFN IVEHKTDPIL LTSMCQPREQ ARESLVSTFR IRPRGRYVS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

709 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.