Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FBXO32 / Fbx32 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G107943-100
Catalog Number: LS-G107943
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Fbx32(FBXO32) (NM_058229), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Atrogin 1 | ATROGIN1 | F-box protein 32 | FBXO32 | F-box only protein 32 | Fbx32 | Muscle atrophy F-box protein | MAFBX | Atrogin-1
- Origin Species
- Human
- NCBI GenBank
- NM_058229
- SwissProt
- Q969P5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 41.5 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MPFLGQDWRS PGQNWVKTAD GWKRFLDEKS GSFVSDLSSY CNKEVYNKEN LFNSLNYDVA61AKKRKKDMLN SKTKTQYFHQ EKWIYVHKGS TKERHGYCTL GEAFNRLDFS TAILDSRRFN121YVVRLLELIA KSQLTSLSGI AQKNFMNILE KVVLKVLEDQ QNIRLIRELL QTLYTSLCTL181VQRVGKSVLV GNINMWVYRM ETILHWQQQL NNIQITRPAF KGLTFTDLPL CLQLNIMQRL241SDGRDLVSLG QAAPDLHVLS EDRLLWKKLC QYHFSERQIR KRLILSDKGQ LDWKKMYFKL301VRCYPRKEQY GDTLQLCKHC HILSWKGTDH PCTANNPESC SVSLSPQDFI NLFKFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
355 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.