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FBXO30 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FBXO30 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FBXO30 antibodies →
$504.00each
Catalog Number: LS-G109032-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FBXO30 (NM_032145), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
F-box protein 30 | F-box domain protein | F-box only protein 30 | FBX30 | FBXO30
Origin Species
Human
NCBI GenBank
NM_032145
SwissProt
Q8TB52

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
82.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEEELQHSHC VNCVSRRCMT RPEPGISCDL IGCPLVCGAV FHSCKADEHR LLCPFERVPC
61LNSDFGCPFT MARNKVAEHL EMCPASVVCC TMEWNRWPVS YADRKSYENL SRDVDEVAQL
121DMALALQDQR MLLESLKVAT MMSKATDKVS KPREQISVKS SVPEIPHANG LVSVDEESYG
181ALYQATVETT RSLAAALDIL NTATRDIGML NTSVPNDMDE QQNARESLED QNLKDQDHLY
241EEEIGAVGGI DYNDTNQNAQ SEQNGSSDLL CDLNTSSYDT SALCNGFPLE NICTQVIDQN
301QNLHGDSKQS NLTNGDCVAS SDGTSKPSSS LAVAAQLREI IPSSALPNGT VQHILMPDDE
361GEGELCWKKV DLGDVKNVDV LSFSHAPSFN FLSNSCWSKP KEDKAVDTSD LEVAEDPMGL
421QGIDLITAAL LFCLGDSPGG RGISDSRMAD IYHIDVGTQT FSLPSAILAT STMVGEIASA
481SACDHANPQL SNPSPFQTLG LDLVLECVAR YQPKQRSMFT FVCGQLFRRK EFSSHFKNVH
541GDIHAGLNGW MEQRCPLAYY GCTYSQRRFC PSIQGAKIIH DRHLRSFGVQ PCVSTVLVEP
601ARNCVLGLHN DHLSSLPFEV LQHIAGFLDG FSLCQLSCVS KLMRDVCGSL LQSRGMVILQ
661WGKRKYPEGN SSWQIKEKVW RFSTAFCSVN EWKFADILSM ADHLKKCSYN VVEKREEAIP
721LPCMCVTREL TKEGRSLRSV LKPVL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

745 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.