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FBXO24 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FBXO24 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FBXO24 antibodies →
$504.00each
Catalog Number: LS-G129067-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FBXO24 (NM_012172), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
F-box protein Fbx24 | FBX24 | FBXO24 | F-box only protein 24 | F-box protein 24
Origin Species
Human
NCBI GenBank
NM_012172

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
69.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVWESQQERG GQGPRRQEGR SREGAIRMVR GSQRRKLQKF TRLCGREEGL RVKRSCPSCG
61SELGVEEKRG KGNPISIQLF PPELVEHIIS FLPVRDLVAL GQTCRYFHEV CDGEGVWRRI
121CRRLSPRLQD QGSGVRPWKR AAILNYTKGL YFQAFGGRRR CLSKSVAPLL AHGYRRFLPT
181KDHVFILDYV GTLFFLKNAL VSTLGQMQWK RACRYVVLCR GAKDFASDPR CDTVYRKYLY
241VLATREPQEV VGTTSSRACD CVEVYLQSSG QRVFKMTFHH SMTFKQIVLV GQETQRALLL
301LTEEGKIYSL VVNETQLDQP RSYTVQLALR KVSHYLPHLR VACMTSNQSS TLYVTDQGGV
361YFEVHTPGVY RDLFGTLQAF DPLDQQMPLA LSLPAKILFC ALGYNHLGLV DEFGRIFMQG
421NNRYGQLGTG DKMDRGEPTQ VCYLQRPITL WCGLNHSLVL SQSSEFSKEL LGCGCGAGGR
481LPGWPKGSAS FVKLQVKVPL CACALCATRE CLYILSSHDI EQHAPYRHLP ASRVVGTPEP
541SLGARAPQDP GGMAQACEEY LSQIHSCQTL QDRTEKMKEI VGWMPLMAAQ KDFFWEALDM
601LQRAEGGGGG VGPPAPET
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

618 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.