Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FBXO15 / FBX15 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G106081-100
Catalog Number: LS-G106081
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Fbx15(FBXO15) (NM_152676), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- F-box only protein 15 | F-box protein 15 | FBX15 | FBXO15
- Origin Species
- Human
- NCBI GenBank
- AA609696
- SwissProt
- Q8NCQ5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 49.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MPSEILLKIF SYLDAVSLLC TGCVSRRFYH LANDNFIWIG IYSTAFSPAR SNWKFNSVEK61IAMSMSFLSV QDKEAGYWKK EYITKQIASV KAALADILKP VNPYTGLPVK TKEALRIFGL121GWAIILKEKG GKEYIMEHVD LSINDTSVTV IWYGKKWPCL ASLSTLDLCG MTPVFTDWYK181TPTKHRLRWH SLIAKYNLSH LTISTMIGCD RLIRIFCLHP GLLVGVWKKE EELAFVMANL241HFHHLVERST LGSATIPYEL PPHSPFLDDS PEYGLHGYQL HVDLHSGGVF YLCGTFRNLF301TKRGNIENGH VKLIVIHLKN NREHLPLIGK VGLSWKTDIF DGCIKSCSMM DVTLLDEHGK361PFWCFSSPVC LRSPATPSDS SSFLGQTYNV DYVDAEGRVH VELVWIRETE EYLIVNLVLY421LSIAKINHWF GTEYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
434 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.