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FBXO11 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FBXO11 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FBXO11 antibodies →
$725.00each
Catalog Number: LS-G101936-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FBXO11 (NM_025133), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
F-box only protein 11 | F-box protein 11 | FBX11 | FBXO11 | PRMT9 | UG063H01 | VIT1 | VIT-1 | UBR6 | Vitiligo-associated protein 1
Origin Species
Human
NCBI GenBank
NM_025133
SwissProt
Q86XK2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
93.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVAEESGPGA QNSPYQLRRK TLLPKRTACP TKNSMEGAST STTENFGHRA KRARVSGKSQ
61DLSAAPAEQY LQEKLPDEVV LKIFSYLLEQ DLCRAACVCK RFSELANDPI LWKRLYMEVF
121EYTRPMMHPE PGKFYQINPE EYEHPNPWKE SFQQLYKGAH VKPGFAEHFY SNPARYKGRE
181NMLYYDTIED ALGGVQEAHF DGLIFVHSGI YTDEWIYIES PITMIGAAPG KVADKVIIEN
241TRDSTFVFME GSEDAYVGYM TIRFNPDDKS AQHHNAHHCL EITVNCSPII DHCIIRSTCT
301VGSAVCVSGQ GACPTIKHCN ISDCENVGLY ITDHAQGIYE DNEISNNALA GIWVKNHGNP
361IIRRNHIHHG RDVGVFTFDH GMGYFESCNI HRNRIAGFEV KAYANPTVVR CEIHHGQTGG
421IYVHEKGRGQ FIENKIYANN FAGVWITSNS DPTIRGNSIF NGNQGGVYIF GDGRGLIEGN
481DIYGNALAGI QIRTNSCPIV RHNKIHDGQH GGIYVHEKGQ GVIEENEVYS NTLAGVWVTT
541GSTPVLRRNR IHSGKQVGVY FYDNGHGVLE DNDIYNHMYS GVQIRTGSNP KIRRNKIWGG
601QNGGILVYNS GLGCIEDNEI FDNAMAGVWI KTDSNPTLRR NKIHDGRDGG ICIFNGGRGL
661LEENDIFRNA QAGVLISTNS HPILRKNRIF DGFAAGIEIT NHATATLEGN QIFNNRFGGL
721FLASGVNVTM KDNKIMNNQD AIEKAVSRGQ CLYKISSYTS YPMHDFYRCH TCNTTDRNAI
781CVNCIKKCHQ GHDVEFIRHD RFFCDCGAGT LSNPCTLAGE PTHDTDTLYD SAPPIESNTL
841QHN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

843 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.