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FBXL7 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FBXL7 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G114529-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FBXL7 (NM_012304), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
FBL6 | F-box protein Fbl7 | F-box/LRR-repeat protein 7 | FBXL7 | F-box protein FBL6/FBL7 | FBL7 | KIAA0840
Origin Species
Human
NCBI GenBank
NM_012304
SwissProt
Q9UJT9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
54.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGANNGKQYG SEGKGSSSIS SDVSSSTDHT PTKAQKNVAT SEDSDLSMRT LSTPSPALIC
61PPNLPGFQNG RGSSTSSSSI TGETVAMVHS PPPTRLTHPL IRLASRPQKE QASIDRLPDH
121SMVQIFSFLP TNQLCRCARV CRRWYNLAWD PRLWRTIRLT GETINVDRAL KVLTRRLCQD
181TPNVCLMLET VTVSGCRRLT DRGLYTIAQC CPELRRLEVS GCYNISNEAV FDVVSLCPNL
241EHLDVSGCSK VTCISLTREA SIKLSPLHGK QISIRYLDMT DCFVLEDEGL HTIAAHCTQL
301THLYLRRCVR LTDEGLRYLV IYCASIKELS VSDCRFVSDF GLREIAKLES RLRYLSIAHC
361GRVTDVGIRY VAKYCSKLRY LNARGCEGIT DHGVEYLAKN CTKLKSLDIG KCPLVSDTGL
421ECLALNCFNL KRLSLKSCES ITGQGLQIVA ANCFDLQTLN VQDCEVSVEA LRFVKRHCKR
481CVIEHTNPAF F
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

491 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.