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FBXL6 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FBXL6 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G114605-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FBXL6 (NM_012162), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
F-box/LRR-repeat protein 6 | FBL6 | FBL6A | F-box protein FBL6 | FBXL6 | PP14630
Origin Species
Human
NCBI GenBank
NM_024555
SwissProt
Q8N531

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
58.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAPASRQVR RRARAAPRPR SAEDWWWDRL APRGSGYHLL QSDSMLLVLS EPGPARPRAQ
61RRASRRTPRQ PPRGPSAAAK PKAGLRSEAA AAPAPAPAPT PTPEEGPDAG WGDRIPLEIL
121VQIFGLLVAA DGPMPFLGRA ARVCRRWQEA ASQPALWHTV TLSSPLVGRP AKGGVKAEKK
181LLASLEWLMP NRFSQLQRLT LIHWKSQVHP VLKLVGECCP RLTFLKLSGC HGVTADALVM
241LAKACCQLHS LDLQHSMVES TAVVSFLEEA GSRMRKLWLT YSSQTTAILG ALLGSCCPQL
301QVLEVSTGIN RNSIPLQLPV EALQKGCPQL QVLRLLNLMW LPKPPGRGVA PGPGFPSLEE
361LCLASSTCNF VSNEVLGRLL HGSPNLRLLD LRGCARITPA GLQDLPCREL EQLHLGLYGT
421SDRLTLAKEG SPFLTQKWCH TLRELDLSGQ GFSEKDLEQA LAAFLSTPGG SHPALCSLNL
481RGTRVTPSTV SSVISSCPGL LYLNLESCRC LPRGLKRAYR GLEEVQWCLE QLLTSPSPS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

539 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.