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FBXL18 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FBXL18 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FBXL18 antibodies →
$725.00each
Catalog Number: LS-G109649-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FBXL18 (NM_024963), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
FBXL18 | Fbl18 | F-box/LRR-repeat protein 18
Origin Species
Human
NCBI GenBank
NM_024963
SwissProt
Q96ME1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
78.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MASSGEDISN DDDDMHPAAA GMADGVHLLG FSDEILLHIL SHVPSTDLIL NVRRTCRKLA
61ALCLDKSLIH TVLLQKDYQA SEDKVRQLVK EIGREIQQLS MAGCYWLPGS TVEHVARCRS
121LVKVNLSGCH LTSLRLSKML SALQHLRSLA IDVSPGFDAS QLSSECKATL SRVRELKQTL
181FTPSYGVVPC CTSLEKLLLY FEILDRTREG AILSGQLMVG QSNVPHYQNL RVFYARLAPG
241YINQEVVRLY LAVLSDRTPQ NLHAFLISVP GSFAESGATK NLLDSMARNV VLDALQLPKS
301WLNGSSLLQH MKFNNPFYFS FSRCTLSGGH LIQQVINGGK DLRSLASLNL SGCVHCLSPD
361SLLRKAEDDI DSSILETLVA SCCNLRHLNL SAAHHHSSEG LGRHLCQLLA RLRHLRSLSL
421PVCSVADSAP RADRAPAQPA MHAVPRGFGK KVRVGVQSCP SPFSGQACPQ PSSVFWSLLK
481NLPFLEHLEL IGSNFSSAMP RNEPAIRNSL PPCSRAQSVG DSEVAAIGQL AFLRHLTLAQ
541LPSVLTGSGL VNIGLQCQQL RSLSLANLGM MGKVVYMPAL SDMLKHCKRL RDLRLEQPYF
601SANAQFFQAL SQCPSLQRLC LVSRSGTLQP DAVLAFMARC LQVVMCHLFT GESLATCKSL
661QQSLLRSFQA ERPALNVVIF PLLHEGLTDV IRDVPLVHLD EITLFKSRVA EEPPNLWW
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

718 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.