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FBXL16 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FBXL16 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G105796-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FBXL16 (NM_153350), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C16orf22 | C380A1.1 | FBXL16 | Fbl16 | C380A1.1 (novel protein) | F-box/LRR-repeat protein 16
Origin Species
Human
SwissProt
Q8N461

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
51.5 kda
Protein Type
Lysate
Amino Acid Sequence
1MSSPGIDGDP KPPCLPRNGL VKLPGQPNGL GAASITKGTP ATKNRPCQPP PPPTLPPPSL
61AAPLSRAALA GGPCTPAGGP ASALAPGHPA ERPPLATDEK ILNGLFWYFS ACEKCVLAQV
121CKAWRRVLYQ PKFWAGLTPV LHAKELYNVL PGGEKEFVNL QGFAARGFEG FCLVGVSDLD
181ICEFIDNYAL SKKGVKAMSL KRSTITDAGL EVMLEQMQGV VRLELSGCND FTEAGLWSSL
241SARITSLSVS DCINVADDAI AAISQLLPNL AELSLQAYHV TDTALAYFTA RQGHSTHTLR
301LLSCWEITNH GVVNVVHSLP NLTALSLSGC SKVTDDGVEL VAENLRKLRS LDLSWCPRIT
361DMALEYVACD LHRLEELVLD RCVRITDTGL SYLSTMSSLR SLYLRWCCQV QDFGLKHLLA
421LGSLRLLSPA GCPLLTTTGL SGLVQLQELE ELELTNCPGA TPELFKYFSQ HLPRCLVIE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

479 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.