Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FBX33 / FBXO33 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G103163-100
Catalog Number: LS-G103163
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FBXO33 (NM_203301), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C14_5247 | Fbx33 | FBXO33 | F-box only protein 33 | F-box protein 33
- Origin Species
- Human
- NCBI GenBank
- D60079
- SwissProt
- Q7Z6M2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 62.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLLFLSVPQP RPPGARTRAG AARVARWRRL RLQQLRRLRG LLRVLRGRPG AGSRRRGRMA61LCGQAAGAAS LPSELIVHIF SFLPAPDRLR ASASCSHWRE CLFYPALWPQ LRICLRVSPA121EQPRLEFLMR KCGWFVRELR VEFAAENYLS GGGPGDGGGA DTGTGGEEVE ALQLSARWLE181VLRTYLELVL CVLVSIRNNR NLQKFSLFGD ISVLQQQGSL SNTYLSKVDP DGKKIKQIQQ241LFEEILSNSR QLKWLSCGFM LEIVTPTSLS SLSNAVANTM EHLSLLDNNI PGNSTLITAV301ELERFVNLHS LALDFCDFTA EMARVLTDSN HVPLQRLSLL VHNVSVMHKS LDNMPNDEHW361KALSRKSTSF RVYIMAFDIK SEDMLKILKP SIPLERIHFD SYITCVSGAI VDLISRQYDK421FLTHFILMND VIDTSGFPDL SDNRNEDPLV LLAWRCTKLS LLAIHGYTVW AHNLIAIARL481RGSDLKVLEV TEESIDFDQG ELADQDVDPV HNLIEQVSLG LGQPWHAVMD IESLSVFTEP541NRHFYREMQS FSEDITRTRPLEQKLISEEDLAANDILDYKDDDDKV
555 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.