Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FBX23 / Tetraspanin 17 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G114612-100
Catalog Number: LS-G114612
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TSPAN17 (NM_012171), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- FBXO23 | F-box only protein 23 | Tetraspanin-17 | Tspan-17 | TM4SF17 | FBX23 | Tetraspan protein SB134 | Tetraspanin 17 | TSPAN17
- Origin Species
- Human
- NCBI GenBank
- NM_012171
- SwissProt
- Q96FV3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 36.9 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MPGKHQHFQE PEVGCCGKYF LFGFNIVFWV LGALFLAIGL WAWGEKGVLS NISALTDLGG61LDPVWLFVVV GGVMSVLGFA GCIGALRENT FLLKFFSVFL GLIFFLELAT GILAFVFKDW121IRDQLNLFIN NNVKAYRDDI DLQNLIDFAQ EYWSCCGARG PNDWNLNIYF NCTDLNPSRE181RCGVPFSCCV RDPAEDVLNT QCGYDVRLKL VRGELEQQGF IHTKGCVGQF EKWLQDNLIV241VAGVFMGIAL LQIFGICLAQ NLVSDIKAVK ANWSKWNDDF ENHWLTPTIS EVLSTAGPQQ301NSLTGAPGPA PPSRHVFFGL GGLYPEPTFK NWTRTRPLEQKLISEEDLAANDILDYKDDDDKV
332 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.