Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FBLN2 / Fibulin 2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G128432-100
Catalog Number: LS-G128432
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Fibulin 2(FBLN2) (NM_001165035), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- FIBL-2 | Fibulin-2 | FBLN2 | Fibulin 2
- Origin Species
- Human
- NCBI GenBank
- NM_001998
- SwissProt
- P98095
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 131.86 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MVLLWEPAGA WLALGLALAL GPSVAAAAPR QDCTGVECPP LENCIEEALE PGACCATCVQ61QGCACEGYQY YDCLQGGFVR GRVPAGQSYF VDFGSTECSC PPGGGKISCQ FMLCPELPPN121CIEAVVVADS CPQCGQVGCV HAGHKYAAGH TVHLPPCRAC HCPDAGGELI CYQLPGCHGN181FSDAEEGDPE RHYEDPYSYD QEVAEVEAAT ALGGEVQAGA VQAGAGGPPA ALGGGSQPLS241TIQAPPWPAV LPRPTAAAAL GPPAPVQAKA RRVTEDSEEE EEEEEEREEM AVTEQLAAGG301HRGLDGLPTT APAGPSLPIQ EERAEAGARA EAGARPEENL ILDAQATSRS TGPEGVTHAP361SLGKAALVPT QAVPGSPRDP VKPSPHNILS TSLPDAAWIP PTREVPRKPQ VLPHSHVEED421TDPNSVHSIP RSSPEGSTKD LIETCCAAGQ QWAIDNDECL EIPESGTEDN VCRTAQRHCC481VSYLQEKSCM AGVLGAKEGE TCGAEDNDSC GISLYKQCCD CCGLGLRVRA EGQSCESNPN541LGYPCNHVML SCCEGEEPLI VPEVRRPPEP AAAPRRVSEA EMAGREALSL GTEAELPNSL601PGDDQDECLL LPGELCQHLC INTVGSYHCA CFPGFSLQDD GRTCRPEGHP PQPEAPQEPA661LKSEFSQVAS NTIPLPLPQP NTCKDNGPCK QVCSTVGGSA ICSCFPGYAI MADGVSCEDQ721DECLMGAHDC SRRQFCVNTL GSFYCVNHTV LCADGYILNA HRKCVDINEC VTDLHTCSRG781EHCVNTLGSF HCYKALTCEP GYALKDGECE DVDECAMGTH TCQPGFLCQN TKGSFYCQAR841QRCMDGFLQD PEGNCVDINE CTSLSEPCRP GFSCINTVGS YTCQRNPLIC ARGYHASDDG901TKCVDVNECE TGVHRCGEGQ VCHNLPGSYR CDCKAGFQRD AFGRGCIDVN ECWASPGRLC961QHTCENTLGS YRCSCASGFL LAADGKRCED VNECEAQRCS QECANIYGSY QCYCRQGYQL1021AEDGHTCTDI DECAQGAGIL CTFRCLNVPG SYQCACPEQG YTMTANGRSC KDVDECALGT1081HNCSEAETCH NIQGSFRCLR FECPPNYVQV SKTKCERTTC HDFLECQNSP ARITHYQLNF1141QTGLLVPAHI FRIGPAPAFT GDTIALNIIK GNEEGYFGTR RLNAYTGVVY LQRAVLEPRD1201FALDVEMKLW RQGSVTTFLA KMHIFFTTFA LTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1231 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.