Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FBLIM1 / Migfilin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G101481-100
Catalog Number: LS-G101481
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FBLIM1 (NM_017556), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CAL | CSX-associated LIM | FBLP1 | Filamin-binding LIM protein 1 | FBLIM1 | Filamin binding LIM protein 1 | Filamin-binding LIM protein-1 | MIG2-interacting protein | RP11-169K16.5 | Migfilin | FBLP-1
- Origin Species
- Human
- SwissProt
- Q8WUP2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 40.5 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MASKPEKRVA SSVFITLAPP RRDVAVAEEV RQAVCEARRG RPWEAPAPMK TPEAGLAGRP61SPWTTPGRAA ATVPAAPMQL FNGGCPPPPP VLDGEDVLPD LDLLPPPPPP PPVLLPSEEE121APAPMGASLI ADLEQLHLSP PPPPPQAPAE GPSVQPGPLR PMEEELPPPP AEPVEKGAST181DICAFCHKTV FPRELAVEAM KRQYHAQCFT CRTCRRQLAG QSFYQKDGRP LCEPCYQDTL241ERCGKCGEVV RDHIIRALGQ AFHPSCFTCV TCARCIGDES FALGSQNEVY CLDDFYRKFA301PVCSICENPI IPRDGKDAFK IECMGRNFHE NCYRCEDCRI LLSVEPTDQG CYPLNNHLFC361KPCHVKRSAA GCCTRTRPLEQKLISEEDLAANDILDYKDDDDKV
373 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.