Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FBL8 / FBXL8 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G111750-100
Catalog Number: LS-G111750
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FBXL8 (NM_018378), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- F-box protein FBL8 | FBXL8 | F-box/LRR-repeat protein 8 | FBL8
- Origin Species
- Human
- NCBI GenBank
- NM_018378
- SwissProt
- Q96CD0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 40.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAEPGEGLPE EVLALIFRHL SLRDRAAAAR VCRAWAAAAT CSAVWHDTKI SCECELEGML61PPYLSACLDH IHNLRLEFEP SRKPSRRAAI ELLMVLAGRA PGLRGLRLEC RGEKPLFDAG121RDVLEAVHAV CGAASQLRHL DLRRLSFTLD DALVLQAARS CPELHSLFLD NSTLVGSVGP181GSVLELLEAC PRLRALGLHL ASLSHAILEA LAAPDRAPFA LLALRCACPE DARASPLPNE241AWVALRRRHP GLAVELELEP ALPAESVTRV LQPAVPVAAL RLNLSGDTVG PVRFAAHHYA301ATLCALEVRA AASAELNAAL EELAARCAAL REVHCFCVVS HSVLDAFRAH CPRLRTYTLK361LTREPHPWRP TLVATRTRPLEQKLISEEDLAANDILDYKDDDDKV
374 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.