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FASTKD5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FASTKD5 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FASTKD5 antibodies →
$504.00each
Catalog Number: LS-G110595-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FASTKD5 (NM_021826), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DJ1187M17.5 | FASTKD5 | FAST kinase domains 5 | KIAA1792
Origin Species
Human
NCBI GenBank
NM_021826
SwissProt
Q7L8L6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
86.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAATLKSLKL VRYRAFCSPS AFGAVRSVSY WNVSSTQHGG QDPPEHISLC HSAKKVKNIC
61STFSSRRILT TSSAHPGLEF SKTSSSKAST LQLGSPRATG VDEEDVEVFD SFENMRVFLQ
121LRPEYRVHSY NASETSQLLS VSEGELILHK VRVNQNNLQA QVIVDYLCKL SSLPAEQHPV
181LLGSTSFALL CQLSVKKIQL FDTQDLINVL KAFVILGIPH SHSMLDVYET KCCHQVWEMN
241MDQLLLVADL WRYLGRKVPR FLNIFSSYLN LHWKDLSLSQ LVHLIYVIGE NRQVSQDLMQ
301KLESLILKYI DLINLEEVGT ICLGFFKSST NLSEFVMRKI GDLACANIQH LSSRSLVNIV
361KMFRFTHVDH INFMKQIGEI APQRIPSLGV QGVMHLTLYC SALRFLNEGV MNAVAASLPP
421RVAHCRSKDV AKILWSFGTL NYKPPNAEEF YSSLISEIHR KMPEFNQYPE HLPTCLLGLA
481FLEYFPVELI DFALSPGFVR LAQERTKFDL LKELYTLDGT VGIECPDYRG NRLSTHLQQE
541GSELLWYLAE KDMNSKPEFL ETVFLLETML GGPQYVKHHM ILPHTRSSDL EVQLDVNLKP
601LPFNREATPA ENVAKLRLEH VGVSLTDDLM NKLLKGKARG HFQGKTESEP GQQPMELENK
661AAVPLGGFLC NVADKSGAME MAGLCPAACM QTPRMKLAVQ FTNRNQYCYG SRDLLGLHNM
721KRRQLARLGY RVVELSYWEW LPLLKRTRLE KLAFLHEKVF TSAL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

764 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.