Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FARP2 / FRG Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G113684-100
Catalog Number: LS-G113684
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FARP2 (NM_014808), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- FRG | FARP2 | FGD1-related Cdc42-GEF | PLEKHC3 | KIAA0793 | FERM domain including RhoGEF | FIR
- Origin Species
- Human
- NCBI GenBank
- NM_014808
- SwissProt
- O94887
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 119.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGEIEGTYRV LQTAGMRLGA QTPVGVSTLE PGQTLLPRMQ EKHLHLRVKL LDNTMEIFDI61EPKCDGQVLL TQVWKRLNLV ECDYFGMEFQ NTQSYWIWLE PMKPIIRQIR RPKNVVLRLA121VKFFPPDPGQ LQEEYTRYLF ALQLKRDLLE ERLTCADTTA ALLTSHLLQS EIGDYDETLD181REHLKVNEYL PGQQHCLEKI LEFHQKHVGQ TPAESDFQVL EIARKLEMYG IRFHMASDRE241GTKIQLAVSH MGVLVFQGTT KINTFNWSKV RKLSFKRKRF LIKLHPEVHG PYQDTLEFLL301GSRDECKNFW KICVEYHTFF RLLDQPKPKA KAVFFSRGSS FRYSGRTQKQ LVDYFKDSGM361KRIPYERRHS KTHTSVRALT ADLPKQSISF PEGLRTPASP SSANAFYSLS PSTLVPSGLP421EFKDSSSSLT DPQVSYVKSP AAERRSGAVA GGPDTPSAQP LGPPALQPGP GLSTKSPQPS481PSSRKSPLSL SPAFQVPLGP AEQGSSPLLS PVLSDAGGAG MDCEEPRHKR VPADEAYFIV541KEILATERTY LKDLEVITVW FRSAVVKEDA MPATLMTLLF SNIDPIYEFH RGFLREVEQR601LALWEGPSKA HTKGSHQRIG DILLRNMRQL KEFTSYFQRH DEVLTELEKA TKRCKKLEAV661YKEFELQKVC YLPLNTFLLK PIQRLLHYRL LLRRLCGHYS PGHHDYADCH DALKAITEVT721TTLQHILIRL ENLQKLTELQ RDLVGIENLI APGREFIREG CLHKLTKKGL QQRMFFLFSD781MLLYTSKGVA GTSHFRIRGL LPLQGMLVEE SDNEWSVPHC FTIYAAQKTI VVAASTRLEK841EKWMLDLNSA IQAAKSGGDT APALPGRTVC TRPPRSPNEV SLEQESEDDA RGVRSSLEGH901GQHRANTTMH VCWYRNTSVS RADHSAAVEN QLSGYLLRKF KNSHGWQKLW VVFTNFCLFF961YKTHQDDYPL ASLPLLGYSV SIPREADGIH KDYVFKLQFK SHVYFFRAES KYTFERWMEV1021IQGASSSAGR APSIVQDGPQ PSSGLEGMVR GKEETRTRPLEQKLISEEDLAANDILDYKDDDDKV
1054 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.