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FAM98A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FAM98A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FAM98A antibodies →
$504.00each
Catalog Number: LS-G113197-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM98A (NM_015475), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DKFZP564F0522 | FAM98A | Protein FAM98A
Origin Species
Human
NCBI GenBank
NM_015475
SwissProt
Q8NCA5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
55.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MECDLMETDI LESLEDLGYK GPLLEDGALS QAVSAGASSP EFTKLCAWLV SELRVLCKLE
61ENVQATNSPS EAEEFQLEVS GLLGEMNCPY LSLTSGDVTK RLLIQKNCLL LLTYLISELE
121AARMLCVNAP PKKAQEGGGS EVFQELKGIC IALGMSKPPA NITMFQFFSG IEKKLKETLA
181KVPPNHVGKP LLKKPMGPAH WEKIEAINQA IANEYEVRRK LLIKRLDVTV QSFGWSDRAK
241SQTEKLAKVY QPKRSVLSPK TTISVAHLLA ARQDLSKILR TSSGSIREKT ACAINKVLMG
301RVPDRGGRPN EIEPPPPEMP PWQKRQDGPQ QQTGGRGGGR GGYEHSSYGG RGGHEQGGGR
361GGRGGYDHGG RGGGRGNKHQ GGWTDGGSGG GGGYQDGGYR DSGFQPGGYH GGHSSGGYQG
421GGYGGFQTSS SYTGSGYQGG GYQQDNGYQD GGHHGDRGGG RGGRGGRGGR GGRAGQGGGW
481GGRGSQNYHQ GGQFEQHFQH GGYQYNHSGF GQGRHYTS
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

518 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.