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FAM83G Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FAM83G Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FAM83G antibodies →
$725.00each
Catalog Number: LS-G120427-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM83G (NM_001039999), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
FAM83G | Protein FAM83G
Origin Species
Human
NCBI GenBank
NM_001039999
SwissProt
A6ND36

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
90.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAFSQVQCLD DNHVNWRSSE SKPEFFYSEE QRLALEALVA RGRDAFYEVL KRENIRDFLS
61ELELKRILET IEVYDPGSED PRGTGPSQGP EDNGVGDGEE ASGADGVPIE AEPLPSLEYW
121PQKSDRSIPQ LDLGWPDTIA YRGVTRASVY MQPPIDGQAH IKEVVRKMIS QAQKVIAVVM
181DMFTDVDIFK DLLDAGFKRK VAVYIIVDES NVKYFLHMCE RACMHLGHLK NLRVRSSGGT
241EFFTRSATKF KGALAQKFMF VDGDRAVCGS YSFTWSAART DRNVISVLSG QVVEMFDRQF
301QELYLMSHSV SLKGIPMEKE PEPEPIVLPS VVPLVPAGTV AKKLVNPKYA LVKAKSVDEI
361AKISSEKQEA KKPLGLKGPA LAEHPGELPE LLPPIHPGLL HLERANMFEY LPTWVEPDPE
421PGSDILGYIN IIDPNIWNPQ PSQMNRIKIR DTSQASAQHQ LWKQSQDSRP RPEPCPPPEP
481SAPQDGVPAE NGLPQGDPEP LPPVPKPRTV PVADVLARDS SDIGWVLELP KEEAPQNGTD
541HRLPRMAGPG HAPLQRQLSV TQDDPESLGV GLPNGLDGVE EEDDDDYVTL SDQDSHSGSS
601GRGPGPRRPS VASSVSEEYF EVREHSVPLR RRHSEQVANG PTPPPRRQLS APHITRGTFV
661GPQGGSPWAQ SRGREEADAL KRMQAQRSTD KEAQGQQFHH HRVPASGTRD KDGFPGPPRY
721RSAADSVQSS TRNAGPAMAG PHHWQAKGGQ VPRLLPDPGS PRLAQNARPM TDGRATEEHP
781SPFGIPYSKL SQSKHLKART GGSQWASSDS KRRAQAPRDR KDP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

823 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.