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FAM83F Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FAM83F Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FAM83F antibodies →
$504.00each
Catalog Number: LS-G107397-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM83F (NM_138435), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
FAM83F | Protein FAM83F
Origin Species
Human
SwissProt
Q8NEG4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
55.3 kda
Protein Type
Lysate
Amino Acid Sequence
1MDLFTDGDIF QDIVDAACKR RVPVYIILDE AGVKYFLEMC QDLQLTDFRI RNIRVRSVTG
61VGFYMPMGRI KGTLSSRFLM VDGDKVATGS YRFTWSSSHV DRNLLLLLTG QNVEPFDTEF
121RELYAISEEV DLYRQLSLAG RVGLHYSSTV ARKLINPKYA LVSGCRHPPG EMMRWAARQQ
181WEAGGNPEGQ EEGASGGESA WRLESFLKDL VTVEQVLPPV EPIPLGELSQ KDGRMVSHMH
241RDLKPKSREA PSRNGMGEAA RGEAAPARRF SSRLFSRRAK RPAAPNGMAS SVSTETSEVE
301FLTGKRPNEN SSADISGKTS PSSAKPSNCV IS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

332 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.