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FAM83C Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FAM83C Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FAM83C antibodies →
$725.00each
Catalog Number: LS-G104680-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM83C (NM_178468), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C20orf128 | DJ614O4.7 | FAM83C | Protein FAM83C
Origin Species
Human
NCBI GenBank
NM_178468
SwissProt
Q9BQN1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
80.9 kda
Protein Type
Lysate
Amino Acid Sequence
1MFGGPGPGVL GAQGMAGPLR GRVEELKLPW WRESSPLVLR HSEAARLAAD ALLERGEAAY
61LRVISEEREL PFLSALDVDY MTSHVRGGPE LSEAQGQEAS GPDRLSLLSE VTSGTYFPMA
121SDIDPPDLDL GWPEVPQATG FSPTQAVVHF QRDKAKNIKD LLRFLFSQAH TVVAVVMDIF
181TDMELLCDLM EASSRRGVPV YLLLAQEHLR HFLEMCYKMD LNGEHLPNMR VRSTCGDTYC
241SKAGRRFTGQ ALEKFVLIDC EQVVAGSYSF TWLCSQAHTS MVLQLRGRIV EDFDREFRCL
301YAESQPVEGF CGGEDPLSPR ALRPPPVALA FRPDVPSPTS SLPSSTSLSS IKQSPLMGRS
361SYLALPGGGD CSDTGVVSSS LGPARREASG QPSLHRQLSD PNHGSPPGLY RANLGKLGAY
421PWSQSSPALN HNSTSPLTLA VGSPLLPRSR PLLQFHRGAP ALSRFPENGL PGSQEPSPLR
481GRWVPGTTLE TVEEKEKKAS PSQSRGQLDL LVPFPRAREV GDPDSGVTPN SGPLRPGEQA
541PEDRRLSPSQ ADSQLDLLSR ALGTGGAPEL GSLRPGDRAL EDRRLSLNQS RGQSDLLMQY
601PKAQGSRVPL ETNSSARPAR RAPDERRQTL GHSQLDLITK FGPFRGEGPG PNGLPISSPA
661RTAGAGSGDE KRLTLGHSKL DLITKYHQLH GARQGTEPGG PKGGHLNGGN SDLVRDEKRL
721TLGHSKLDLI TKYNKSKFKQ LRSRFES
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

747 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.