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FAM82A1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FAM82A1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all RMDN2 antibodies →
$725.00each
Catalog Number: LS-G106878-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM82A1(RMDN2) (NM_144713), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BLOCK18 | FAM82A | PYST9371 | RMD-2 | RMD2 | RMD4 | FAM82A1 | PRO34163 | RMDN2
Origin Species
Human
SwissProt
Q96LZ7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
65 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGKCLSCCKE DQSFQRCSPE DQVSTDAQHR GASSISQPSI SLGHKTSYSP VTHKVNAAKA
61SRRLLSVSSP SFSERRYSLF VGFQKRNASP YWQQSRANFD SEEDTGFTDI KSSSDHCGSF
121ISRRRRFSSR KLSIVSYYKS AIFFDPQASG QNVFNLNEIE IFSKTSSNTD AKKHITISAP
181EYNTKNFKNF ETNTTSPAFG NTIDTASYQQ STSSFFSLAS DISSPDQQNG IANDIQQRGQ
241LCKDLKDFLH PRPESYSTGH SPIMIPQHPS QSGTFPFLHK AGFSSSYKNS GCFIPPQSEL
301TSGLFEDEDF AVLFQDEDRS SPIEIPKIRY ITANTDTEEQ SFPVPKAFNT RVEELNLDVL
361LQKVDHLRMS ESGKSESFEL LRDHKEKFRD EIEFMWRFAR AYGDMYELST NTQEKKHYAN
421IGKTLSERAI NRAPMNGHCH LWYAVLCGYV SEFEGLQNKI NYGHLFKEHL DIAIKLLPEE
481PFLYYLKGRY CYTVSKLSWI EKKMAATLFG KIPSSTVQEA LHNFLKAEEL CPGYSNPNYM
541YLAKCYTDLE ENQNALKFCN LALLLPTVTK EEL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

573 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.