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FAM71D Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FAM71D Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FAM71D antibodies →
$725.00each
Catalog Number: LS-G105193-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM71D (NM_173526), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C14orf54 | FAM71D | Protein FAM71D
Origin Species
Human
SwissProt
Q8N9W8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
46.9 kda
Protein Type
Lysate
Amino Acid Sequence
1MKKNTSKTTM RINKQDALCT PHSHDPRDLQ NMLDGGEYAP FVSPPMLESN FIQVNRRGES
61IYLHNRANWV TVGICFSSST HKIPNVMLLA HLTPGAQKDT ETLFKSLLTS PPAEKLVLTR
121FLPLQFVTLS VHDAENMSLK VKLVSGRAYY LQLCTSAYKQ DTLFSQWVAL ISLLNQEKAK
181VSKVSEVSSL SGITNSTDIT GSMDVTDVTT FTAILTPYMY AGTGPEHVRD SIDFPEFTDI
241TDITDVTDLP ENEVPEVPDV RIVTEVIEVR EATEVTDSSD ITNCSGVTVV FENNDLIRAK
301QEEKEKLKNI LKPGCLQDTK SKSELKESSK HVTISNITLT FEGKRYFQTT LTPVESEANT
361SKEMKDKTSE EKMPDFQSTA LKAEESRSLR TESNTSVLSP HIKSPSNFLK LVPHLSAPFS
421RE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

422 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.