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FAM71A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FAM71A Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FAM71A antibodies →
$504.00each
Catalog Number: LS-G105751-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM71A (NM_153606), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
FAM71A | Protein FAM71A | RP11-338C15.4
Origin Species
Human
SwissProt
Q8IYT1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
63 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNADFLLPYY TAQSGSSMSM FDTTMGKLQR QLYKGEYDIF KYAPIFESDF IQITKRGEVI
61DVHNRVRMVT MGIARTSPIL PLPDVMLLAR PATGCEEYAG HGQATKRKKR KAAKNLELTR
121LLPLRFVRIS VQDHEKQQLR LKFATGRSCY LQLCPALDTR DDLFAYWEKL IYLLRPPMES
181NSSTCGIPAE DMMWMPVFQE DRRSLGAVNL QGKGDQDQVS IQSLHMVSEV CGATSAAYAG
241GEGLQNDFNK PTNVLNASIP KTSTELAEEP ATGGIKEAAA AGAAAGAATG TVAGALSVAA
301ANSAPGQVSA AIAGAATIDA GGNKGNMALA GTASMAPNST KVAVAGAAGK SSEHVSSASM
361SLSREGSVSL AIAGVVLTSR TAAEADMDAA AGPPVSTRQS KSSLSGQHGR ERTQASAEGC
421KEGRERREKD RALGRSSHRR RTGESRHKTR GDKIAQKSSS RSSFSHRANR DDKKEKGCGN
481PGSSRHRDSH KGVSHTPISK ESRTSHKSGR SLWTTSSGSS KGLGRVSSFL RNVRANLTTK
541VVGTPHGRDV DVMAKMAERS TNVAIAETAE GGQGLEMVGS MTPDIMETVT FEAH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

594 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.