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FAM65A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FAM65A Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FAM65A antibodies →
$504.00each
Catalog Number: LS-G109952-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM65A (NM_024519), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1930 | Protein FAM65A | FAM65A
Origin Species
Human
NCBI GenBank
NM_024519
SwissProt
Q6ZS17

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
131.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MMSLSVRPQR RLLSARVNRS QSFAGVLGSH ERGPRSFPVF SPPGPPRKPP ALSRVSRMFS
61VAHPAAKVPQ PERLDLVYTA LKRGLTAYLE VHQQEQEKLQ GQIRESKRNS RLGFLYDLDK
121QVKSIERFLR RLEFHASKID ELYEAYCVQR RLRDGAYNMV RAYTTGSPGS REARDSLAEA
181TRGHREYTES MCLLESELEA QLGEFHLRMK GLAGFARLCV GDQYEICMKY GRQRWKLRGR
241IEGSGKQVWD SEETIFLPLL TEFLSIKVTE LKGLANHVVV GSVSCETKDL FAALPQVVAV
301DINDLGTIKL SLEVTWSPFD KDDQPSAASS VNKASTVTKR FSTYSQSPPD TPSLREQAFY
361NMLRRQEELE NGTAWSLSSE SSDDSSSPQL SGTARHSPAP RPLVQQPEPL PIQVAFRRPE
421TPSSGPLDEE GAVAPVLANG HAPYSRTLSH ISEASVDAAL AEASVEAVGP ESLAWGPSPP
481THPAPTHGEH PSPVPPALDP GHSATSSTLG TTGSVPTSTD PAPSAHLDSV HKSTDSGPSE
541LPGPTHTTTG STYSAITTTH SAPSPLTHTT TGSTHKPIIS TLTTTGPTLN IIGPVQTTTS
601PTHTMPSPTH TTASPTHTST SPTHTPTSPT HKTSMSPPTT TSPTPSGMGL VQTATSPTHP
661TTSPTHPTTS PILINVSPST SLELATLSSP SKHSDPTLPG TDSLPCSPPV SNSYTQADPM
721APRTPHPSPA HSSRKPLTSP APDPSESTVQ SLSPTPSPPT PAPQHSDLCL AMAVQTPVPT
781AAGGSGDRSL EEALGALMAA LDDYRGQFPE LQGLEQEVTR LESLLMQRQG LTRSRASSLS
841ITVEHALESF SFLNEDEDED NDVPGDRPPS SPEAGAEDSI DSPSARPLST GCPALDAALV
901RHLYHCSRLL LKLGTFGPLR CQEAWALERL LREARVLEAV CEFSRRWEIP ASSAQEVVQF
961SASRPGFLTF WDQCTERLSC FLCPVERVLL TFCNQYGARL SLRQPGLAEA VCVKFLEDAL
1021GQKLPRRPQP GPGEQLTVFQ FWSFVETLDS PTMEAYVTET AEEVLLVRNL NSDDQAVVLK
1081ALRLAPEGRL RRDGLRALSS LLVHGNNKVM AAVSTQLRSL SLGPTFRERA LLCFLDQLED
1141EDVQTRVAGC LALGCIKAPE GIEPLVYLCQ TDTEAVREAA RQSLQQCGEE GQSAHRRLEE
1201SLDALPRIFG PGSMASTAF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1219 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.