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FAM63B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FAM63B Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FAM63B antibodies →
$725.00each
Catalog Number: LS-G120332-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM63B (NM_001040450), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
FAM63B | KIAA1164 | Protein FAM63B
Origin Species
Human
NCBI GenBank
NM_001040450
SwissProt
Q8NBR6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
66.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MESSPESLQP LEHGVAAGPA SGTGSSQEGL QETRLAAGDG PGVWAAETSG GNGLGAAAAR
61RSLPDSASPA GSPEVPGPCS SSAGLDLKDS GLESPAAAEA PLRGQYKVTA SPETAVAGVG
121HELGTAGDAG ARPDLAGTCQ AELTAAGSEE PSSAGGLSSS CSDPSPPGES PSLDSLESFS
181NLHSFPSSCE FNSEEGAENR VPEEEEGAAV LPGAVPLCKE EEGEETAQVL AASKERFPGQ
241SVYHIKWIQW KEENTPIITQ NENGPCPLLA ILNVLLLAWK VKLPPMMEII TAEQLMEYLG
301DYMLDAKPKE ISEIQRLNYE QNMSDAMAIL HKLQTGLDVN VRFTGVRVFE YTPECIVFDL
361LDIPLYHGWL VDPQIDDIVK AVGNCSYNQL VEKIISCKQS DNSELVSEGF VAEQFLNNTA
421TQLTYHGLCE LTSTVQEGEL CVFFRNNHFS TMTKYKGQLY LLVTDQGFLT EEKVVWESLH
481NVDGDGNFCD SEFHLRPPSD PETVYKGQQD QIDQDYLMAL SLQQEQQSQE INWEQIPEGI
541SDLELAKKLQ EEEDRRASQY YQEQEQAAAA AAAASTQAQQ GQPAQASPSS GRQSGNSERK
601RKEPREKDKE KEKEKNSCVI L
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

621 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.