Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FAM62C / ESYT3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G109135-100
Catalog Number: LS-G109135
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM62C(ESYT3) (NM_031913), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Chr3 synaptotagmin | CHR3SYT | E-Syt3 | ESYT3 | Extended synaptotagmin-3 | FAM62C
- Origin Species
- Human
- NCBI GenBank
- NM_031913
- SwissProt
- A0FGR9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 99.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MRAEEPCAPG APSALGAQRT PGPELRLSSQ LLPELCTFVV RVLFYLGPVY LAGYLGLSIT61WLLLGALLWM WWRRNRRGKL GRLAAAFEFL DNEREFISRE LRGQHLPAWI HFPDVERVEW121ANKIISQTWP YLSMIMESKF REKLEPKIRE KSIHLRTFTF TKLYFGQKCP RVNGVKAHTN181TCNRRRVTVD LQICYIGDCE ISVELQKIQA GVNGIQLQGT LRVILEPLLV DKPFVGAVTV241FFLQKPHLQI NWTGLTNLLD APGINDVSDS LLEDLIATHL VLPNRVTVPV KKGLDLTNLR301FPLPCGVIRV HLLEAEQLAQ KDNFLGLRGK SDPYAKVSIG LQHFRSRTIY RNLNPTWNEV361FEFMVYEVPG QDLEVDLYDE DTDRDDFLGS LQICLGDVMT NRVVDEWFVL NDTTSGRLHL421RLEWLSLLTD QEVLTEDHGG LSTAILVVFL ESACNLPRNP FDYLNGEYRA KKLSRFARNK481VSKDPSSYVK LSVGKKTHTS KTCPHNKDPV WSQVFSFFVH NVATERLHLK VLDDDQECAL541GMLEVPLCQI LPYADLTLEQ RFQLDHSGLD SLISMRLVLR FLQVEERELG SPYTGPEALK601KGPLLIKKVA TNQGPKAQPQ EEGPTDLPCP PDPASDTKDV SRSTTTTTSA TTVATEPTSQ661ETGPEPKGKD SAKRFCEPIG EKKSPATIFL TVPGPHSPGP IKSPRPMKCP ASPFAWPPKR721LAPSMSSLNS LASSCFDLAD ISLNIEGGDL RRRQLGEIQL TVRYVCLRRC LSVLINGCRN781LTPCTSSGAD PYVRVYLLPE RKWACRKKTS VKRKTLEPLF DETFEFFVPM EEVKKRSLDV841AVKNSRPLGS HRRKELGKVL IDLSKEDLIK GFSQWYELTP NGQPRSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
886 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.