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FAM55B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FAM55B Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all NXPE2 antibodies →
$504.00each
Catalog Number: LS-G127588-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NXPE2 (NM_182495), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
FAM55B | NXPE family member 2 | NXPE2 | Protein FAM55B
Origin Species
Human
NCBI GenBank
NM_182495
SwissProt
Q96DL1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
64.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVEKILIHRI LTLFPNAIAR KLLLMLTFIL IFWIIYLASK DHTKFSFNLE NHIILNQGNI
61FKKYSHSETP LCPAVSPKET ELRIKDIMEK LDQQIPPRPF THVNTTTSAT HSTATILNPQ
121DTYCRGDQLD ILLEVRDHLG HRKQYGGDFL RARMYSTALM AGASGKVTDF NNGTYLVSFT
181LFWEGQVSLS LLLIHPSEGV SALWRARNQG CDRIIFTGLF ANRSSNVFTE CGLTLNTNAE
241LCQYMDDRDQ EAFYCVRPQH MPCEALTHMT TRTRNISYLS KEEWRLFHRS NIGVEMMKNF
301TPIEVIPCNK SENIKKNCQI GMKTPFPSGY TLKKMWITAF CKQIKFNETK NINDCLERKL
361IYLMGDSTLH QWIYYLQKAV KTLKYFDHHG AGIFKTHVLL DVERHILIQW KKHGHPFVTK
421KLFSVKDENY IPREIDQVAG DKNTAIVITL GQHFRPFPIN IFIRRAINIQ KAIERLFLRS
481PETKVILKTE NTREIEQNAE MFSDFHGYIQ NLIIRDIFVD LNVGIIDAWD MTIAYCTNNA
541HPPDYVIQNQ IGMFLNYIC
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

559 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.