Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FAM54A / DUFD1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G107384-100
Catalog Number: LS-G107384
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM54A(MTFR2) (NM_138419), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DUFD1 | FAM54A | MTFR2
- Origin Species
- Human
- SwissProt
- Q6P444
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 43.2 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MSLILNILRE MLEYFGVPVE QVLLIWENKD YGSTRSIVRI IGKMLPLEPC RRPNFELIPL61LNSVDSDNCG SMVPSFADIL YVANDEEASY LRFRNSIWKN EEEKVEIFHP LRLVRDPLSP121AVRQKETVKN DLPVNEAAIR KIAALENELT FLRSQIAAIV EMQELKNSTN SSSFGLSDER181ISLGQLSSSR AAHLSVDPDQ LPGSVLSPPP PPPLPPQFSS LQPPCFPPVQ PGSNNICDSD241NPATEMSKQN PAANKTNYSH HSKSQRNKDI PNMLDVLKDM NKVKLRAIER SPGGRPIHKR301KRQNSHWDPV SLISHALKQK FAFQEDDSFE KENRSWESSP FSSPETSRFG HHISQSEGQR361TKEEMVNTKA VDQGISNTSL LNSRITRTRPLEQKLISEEDLAANDILDYKDDDDKV
385 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.