LSBio
FAM50B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FAM50B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FAM50B antibodies →
$504.00each
Catalog Number: LS-G114625-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM50B (NM_012135), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
D6S2654E | FAM50B | X5L | Protein FAM50B | Protein XAP-5-like
Origin Species
Human
NCBI GenBank
NM_012135
SwissProt
Q9Y247

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
38.5 kda
Protein Type
Lysate
Amino Acid Sequence
1MAQYKGTMRE AGRAMHLLKK RERQREQMEV LKQRIAEETI LKSQVDKRFS AHYDAVEAEL
61KSSTVGLVTL NDMKARQEAL VRERERQLAK RQHLEEQRLQ QERQREQEQR RERKRKISCL
121SFALDDLDDQ ADAAEARRAG NLGKNPDVDT SFLPDRDREE EENRLREELR QEWEAQREKV
181KDEEMEVTFS YWDGSGHRRT VRVRKGNTVQ QFLKKALQGL RKDFLELRSA GVEQLMFIKE
241DLILPHYHTF YDFIIARARG KSGPLFSFDV HDDVRLLSDA TMEKDESHAG KVVLRSWYEK
301NKHIFPASRW EAYDPEKKWD KYTIRTRTRP LEQKLISEED LAANDILDYK DDDDKV

356 aa

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.