Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FAM49B / L1 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G112549-100
Catalog Number: LS-G112549
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM49B (NM_016623), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BM-009 | Protein FAM49B | FAM49B | L1
- Origin Species
- Human
- NCBI GenBank
- NM_016623
- SwissProt
- Q9NUQ9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 36.6 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MGNLLKVLTC TDLEQGPNFF LDFENAQPTE SEKEIYNQVN VVLKDAEGIL EDLQSYRGAG61HEIREAIQHP ADEKLQEKAW GAVVPLVGKL KKFYEFSQRL EAALRGLLGA LTSTPYSPTQ121HLEREQALAK QFAEILHFTL RFDELKMTNP AIQNDFSYYR RTLSRMRINN VPAEGENEVN181NELANRMSLF YAEATPMLKT LSDATTKFVS ENKNLPIENT TDCLSTMASV CRVMLETPEY241RSRFTNEETV SFCLRVMVGV IILYDHVHPV GAFAKTSKID MKGCIKVLKD QPPNSVEGLL301NALRYTTKHL NDETTSKQIK SMLQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
324 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.