Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FAM48A / P38IP Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G121564-100
Catalog Number: LS-G121564
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM48A(SUPT20H) (NM_001014286), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BA421P11.4 | C13orf19 | FP757 | p38-interacting protein | Protein FAM48A | SPT20 | SUPT20H | Tumor rejection antigen | p38IP | C13 | FAM48A | p38 interacting protein
- Origin Species
- Human
- NCBI GenBank
- NM_017569
- SwissProt
- Q8NEM7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 85.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MQQALELALD RAEYVIESAR QRPPKRKYLS SGRKSVFQKL YDLYIEECEK EPEVKKLRRN61VNLLEKLVMQ ETLSCLVVNL YPGNEGYSLM LRGKNGSDSE TIRLPYEEGE LLEYLDAEEL121PPILVDLLEK SQVNIFHCGC VIAEIRDYRQ SSNMKSPGYQ SRHILLRPTM QTLICDVHSI181TSDNHKWTQE DKLLLESQLI LATAEPLCLD PSIAVTCTAN RLLYNKQKMN TRPMKRCFKR241YSRSSLNRQQ DLSHCPPPPQ LRLLDFLQKR KERKAGQHYD LKISKAGNCV DMWKRSPCNL301AIPSEVDVEK YAKVEKSIKS DDSQPTVWPA HDVKDDYVFE CEAGTQYQKT KLTILQSLGD361PLYYGKIQPC KADEESDSQM SPSHSSTDDH SNWFIIGSKT DAERVVNQYQ ELVQNEAKCP421VKMSHSSSGS ASLSQVSPGK ETDQTETVSV QSSVLGKGVK HRPPPIKLPS SSGNSSSGNY481FTPQQTSSFL KSPTPPPSSK PSSIPRKSSV DLNQVSMLSP AALSPASSSQ RTTATQVMAN541SAGLNFINVV GSVCGAQALM SGSNPMLGCN TGAITPAGIN LSGLLPSGGL LPNALPSAMQ601AASQAGVPFG LKNTSSLRPL NLLQLPGGSL IFNTLQQQQQ QLSQFTPQQP QQPTTCSPQQ661PGEQGSEQGS TSQEQALSAQ QAAVINLTGV GSFMQSQAAV LSQLGSVENR PEQSLPQQRF721QLSSAFQQQQ QQIQQLRFLQ HQMAMAAAAA QTAQLHHHRH TGSQSKSKMK RGTPTTPKFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
779 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.