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FAM47E Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FAM47E Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FAM47E antibodies →
$504.00each
Catalog Number: LS-G125767-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM47E (NM_001136570), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
protein FAM47E
Origin Species
Human
NCBI GenBank
NM_001136570
SwissProt
Q6ZV65

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
45.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MADRRRRLRP GTLAPVREGV NCRSRCFTKH KNGLKFPTSL HSRQLVFPRK GLDDFRKGCP
61PCTGLVTQVP VEGFLPQIYH RAPQLAPKKR QIKLLKEADV LSKLSPAQQA RKAFLEDVEA
121HLTPHPLALY LNLEEAMPIE LLSKVLEVLD PDRKLEDTWA YCQDTRKGMK EPTKLLKKHS
181TQVYLGPSKK TSVSNAGQWL YEEKPHKMDL LHENGPRPGL HENGISDIDE EFILKQFDID
241YETKPSHDAL HTMKLNQVPL ELKRSVGLSK LQETEFFQKL GYERKLQKPQ NPYKPKWVKM
301RYGAWYLNPK LWKKQRVDEP LVDPEVSHKA QEENFKKELQ EQEELLADLH GTVAFKDFIL
361SRGYRTPRFL ENMYIGKECK RACNKTPIKR TQA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

393 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.