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FAM40B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FAM40B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G110885-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human STRIP2 (NM_020704), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
FAR11B | STRIP2 | FAM40B | Striatin interacting protein 2
Origin Species
Human
NCBI GenBank
AB032996
SwissProt
Q9ULQ0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
95.2 kda
Protein Type
Lysate
Amino Acid Sequence
1MEDPAAPGTG GPPANGNGNG GGKGKQAAPK GREAFRSQRR ESEGSVDCPT LEFEYGDADG
61HAAELSELYS YTENLEFTNN RRCFEEDFKT QVQGKEWLEL EEDAQKAYIM GLLDRLEVVS
121RERRLKVARA VLYLAQGTFG ECDSEVDVLH WSRYNCFLLY QMGTFSTFLE LLHMEIDNSQ
181ACSSALRKPA VSIADSTELR VLLSVMYLMV ENIRLERETD PCGWRTARET FRTELSFSMH
241NEEPFALLLF SMVTKFCSGL APHFPIKKVL LLLWKVVMFT LGGFEHLQTL KVQKRAELGL
301PPLAEDSIQV VKSMRAASPP SYTLDLGESQ LAPPPSKLRG RRGSRRQLLT KQDSLDIYNE
361RDLFKTEEPA TEEEEESAGD GERTLDGELD LLEQDPLVPP PPSQAPLSAE RVAFPKGLPW
421APKVRQKDIE HFLEMSRNKF IGFTLGQDTD TLVGLPRPIH ESVKTLKQHK YISIADVQIK
481NEEELEKCPM SLGEEVVPET PCEILYQGML YSLPQYMIAL LKILLAAAPT SKAKTDSINI
541LADVLPEEMP ITVLQSMKLG IDVNRHKEII VKSISTLLLL LLKHFKLNHI YQFEYVSQHL
601VFANCIPLIL KFFNQNILSY ITAKNSISVL DYPCCTIQDL PELTTESLEA GDNSQFCWRN
661LFSCINLLRL LNKLTKWKHS RTMMLVVFKS APILKRALKV KQAMLQLYVL KLLKLQTKYL
721GRQWRKSNMK TMSAIYQKVR HRMNDDWAYG NDIDARPWDF QAEECTLRAN IEAFNSRRYD
781RPQDSEFSPV DNCLQSVLGQ RLDLPEDFHY SYELWLEREV FSQPICWEEL LQNH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

834 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.