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FAM35A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FAM35A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FAM35A antibodies →
$504.00each
Catalog Number: LS-G111474-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM35A(FAM35A) (NM_019054), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
FAM35A | Protein FAM35A | BA163M19.1 | FAM35A1
Origin Species
Human
NCBI GenBank
NM_019054
SwissProt
Q86V20

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
93.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSGGSQVHIF WGAPIAPLKI TVSEDTASLM SVADPWKKIQ LLYSQHSLYL KDEKQHKNLE
61NYKVPESIGS PDLSGHFLAN CMNRHVHVKD DFVRSVSETQ NIESQKIHSS RLSDITSSNM
121QICGFKSTVP HFTEEEKYQK LLSENKIRDE QPKHQPDICG KNFNTNLFQL GHKCAAVLDL
181VCSTEKINIG PEVVQRECVP TEYHEIQNQC LGLFSSNAVD KSRSEAAVRK VSDLKISTDT
241EFLSIITSSQ VAFLAQKKDK RRSPVNKGNV NMETEPKASY GEIRIPEENS IQLDGFTEAY
301ESGQNQAYSL ELFSPVCPKT ENSRIHINSD KGLEEHTGSQ ELFSSEDELP PNEIRIELCS
361SGILCSQLNT FHKSAIKRSC TSEDKVGQSE ALSRVLQVAK KMKLISNGGD SAVEMDRRNV
421SEFKSIKKTS LIKNCDSKSQ KYNCLVMVLS PCHVKEINIK FGPNSGSKVP LATVTVIDQS
481ETKKKVFLWR TAAFWAFTVF LGDIILLTDV VIHEDQWIGE TVLQSTFSSQ LLNLGSYSSI
541QPEEYSSVVS EVVLQDLLAY VSSKHSYLRD LPPRQPQRVN SIDFVELEHL QPDVLVHAVL
601RVVDFTILTE AVYSYRGQKQ KKVMLTVEQA QDQHYALVLW GPGAAWYPQL QRKKGVVLIK
661AQISELAFPI TASQKIALNA HSSLKSIFSS LPNIVYTGCA KCGLELETDE NRIYKQCFSC
721LPFTMKKIYY RPALMTAIDG RHDVCIRVES KLIEKILLNI SADCLNRVIV PSSEITYGMV
781VADLFHSLLA VSAEPCVLKI QSLFVLDENS YPLQQDFSLL DFYPDIVKHG ANARL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

835 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.