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FAM227A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FAM227A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FAM227A antibodies →
$504.00each
Catalog Number: LS-G128238-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM227A (NM_001013647), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_001013647

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
66 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNHFRKMEVI NLTTLPMIPV DEHLAVSLVA RNTMVKTVRK ELENNPPSCL IGSMHQVNQK
61IADINLRTEP SANSLAIERF ELEKKALREK TRSSPEDKVK RQRKSQYSCK GSELRHARSS
121VIKRKTADKN LLAELYQYSN FNSSKPNKLP NGVDFCDMVG NVVRAERDCL SGKHFCSGRE
181LEKFLSSSSP RAIWLDSFWW IFHERYQPNK ELQNNLFDRI AQHYALLLFR VPKSHSEEAL
241LKRLPSLLSK AVYTSFCCCF PQSWFDTHEF KSDICNTMSL WISGTYPSPQ SYDSWDYSEL
301DPERFRREEL MLYRRRLTKG REFSLFAGKR AFSQKPAQSR KFYHPQSSSA NSPSEKTSSA
361KQNSEKSLRM QNTAKEHHCQ TLVLKKPTQE VKRISEAREC ENMFPKKSCA ACKSPELTSN
421LFNIYGKSPL IVYFLQNYAS LQQHGKNVLI VRREKTTSTP DCTPTYTDVI SETLCSMKKR
481KDNLNQLYQH HWTEWNYFDK HLKELQDNFS REMKNIDPKA ADTKKANHMF IPPSAVNEES
541PDKKTKEGKG GEGKRRETEV EHFFPLTSKP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

570 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.