LSBio
FAM222B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FAM222B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FAM222B antibodies →
$504.00each
Catalog Number: LS-G111903-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM222B (NM_018182), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C17orf63 | FAM222B | Protein FAM222B
Origin Species
Human
NCBI GenBank
NM_018182
SwissProt
Q8WU58

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
59.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLACLPGPGD LSFQLLSHTQ MNTGLQKWDT TQKMRTAHYP TPAELDAYAK KVANNPLTIK
61IFPNSVKVPQ RKHVRRTVNG LDTSAQRYSP YPTQAATKAG LLAIVKVPAK SILKDFDGTR
121ARLLPEAIMN PPVAPYATVA PSTLAHPQAQ ALARQQALQH AQTLAHAPPQ TLQHPQGIPP
181PQALSHPQSL QQPQGLGHPQ PMAQTQGLVH PQALAHQGLQ HPHNPLLHGG RKMPDSDAPP
241NVTVSTSTIP LSMAATLQHS QPPDLSSIVH QINQFCQTRA GISTTSVCEG QIANPSPISR
301SLLINASTRV STHSVPTPMP SCVVNPMEHT HAATAALPAA GPVNLPTGIS RVPTGYPSDL
361KPVTWNQHQL AHLQQMCSEA SGTPAPGLTG KHAAGRELAG PGFVGKAPAY PQELCLAQSF
421HLKPPLEKPT PSPPVNGMAA PLAYPNGHYF QPLWNNILPT PNSDSSGSQD LAMPFHGGQP
481TGAPLDCAAA PGAHYRAGTG GGPVASQNSL MQTVDYLSGD FQQACFREQS LAMLSKAHRA
541PGNRAPDPTE SRSLHIQHPG YR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

562 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.