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FAM20C Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FAM20C Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FAM20C antibodies →
$504.00each
Catalog Number: LS-G111266-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM20C (NM_020223), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Dentin matrix protein 4 | DMP4 | FAM20C | GEF-CK | IMAGE:4942737 | Protein FAM20C | RNS | DMP-4
Origin Species
Human
SwissProt
Q8IXL6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
66.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVFLVACALH IALDLLPRLE RRGARPSGEP GCSCAQPAAE VAAPGWAQVR GRPGEPPAAS
61SAAGDAGWPN KHTLRILQDF SSDPSSNLSS HSLEKLPPAA EPAERALRGR DPGALRPHDP
121AHRPLLRDPG PRRSESPPGP GGDASLLARL FEHPLYRVAV PPLTEEDVLF NVNSDTRLSP
181KAAENPDWPH AGAEGAEFLS PGEAAVDSYP NWLKFHIGIN RYELYSRHNP AIEALLHDLS
241SQRITSVAMK SGGTQLKLIM TFQNYGQALF KPMKQTREQE TPPDFFYFSD YERHNAEIAA
301FHLDRILDFR RVPPVAGRMV NMTKEIRDVT RDKKLWRTFF ISPANNICFY GECSYYCSTE
361HALCGKPDQI EGSLAAFLPD LSLAKRKTWR NPWRRSYHKR KKAEWEVDPD YCEEVKQTPP
421YDSSHRILDV MDMTIFDFLM GNMDRHHYET FEKFGNETFI IHLDNGRGFG KYSHDELSIL
481VPLQQCCRIR KSTYLRLQLL AKEEYKLSLL MAESLRGDQV APVLYQPHLE ALDRRLRVVL
541KAVRDCVERN GLHSVVDDDL DTEHRAASAR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

570 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.