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FAM200B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FAM200B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FAM200B antibodies →
$504.00each
Catalog Number: LS-G126573-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM200B (NM_001145191), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_001145191

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
75.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDHFFIKRKR NSEVKYTEAC SSSSVESGIV NSDNIEKNTD SNLQTSTSFE PHFKKKKVSA
61RRYNEDYLKY GFIKCEKPFE NDRPQCVICN NILANESLKP SKLKRHLETQ HAELIDKPLE
121YFQRKKKDIK LSTQFLSCST AVSEKALLSS YLVAYRVAKE KIANTAAEKI ILPACLDMVR
181TIFDDKSADK LKTIPNDNTV SLRICTIAEH LETMLITRLQ SGIDFAIQLD ESTDIGSCTT
241LLVYVRYAWQ DDFLEDFLCF LNLTSHLSGL DIFTELERRI VGQYKLNWKN CKGITSDGTA
301TMTGKHSRVI KKLLEVTNNG AVWNHCFIHR EGLASREIPQ NLMEVLKNAV KVVNFIKGSS
361LNSRLLETFC SEIGTNHTHL LYHTKIRWLS QGKILSRVYE LRNEIHFFLI EKKSHLASIF
421EDDTWVTKLA YLTDIFSILN ELSLKLQGKN SDVFQHVERI QGFRKTLLLW QVRLKSNRPS
481YYMFPRFLQH IEENIINENI LKEIKLEILL HLTSLSQTFN HFFPEEKFET LRENSWVKDP
541FAFRHPESII ELNLVPEEEN ELLQLSSSYT LKNDYETLSL SAFWMKVKED FPLLSRKSVL
601LLLPFTTTSL CELGFSILTQ LKTKERNGLN CAAVMRVALS SCVPDWNELM NRQAHPS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

657 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.