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FAM200A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FAM200A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FAM200A antibodies →
$504.00each
Catalog Number: LS-G106732-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM200A (NM_145111), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C7orf38 | FAM200A | Protein FAM200A
Origin Species
Human
SwissProt
Q8TCP9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
66.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTPESRDTTD LSPGGTQEME GIVIVKVEEE DEEDHFQKER NKVESSPQVL SRSTTMNERA
61LLSSYLVAYR VAKEKMAHTA AEKIILPACM DMVRTIFDDK SADKLRTIPL SDNTISRRIC
121TIAKHLEAML ITRLQSGIDF AIQLDESTDI ASCPTLLVYV RYVWQDDFVE DLLCCLNLNS
181HITGLDLFTE LENCLLGQYK LNWKHCKGIS SDGTANMTGK HSRLTEKLLE ATHNNAVWNH
241CFIHREALVS KEISPSLMDV LKNAVKTVNF IKGSSLNSRL LEIFCSEIGV NHTHLLFHTE
301VRWLSQGKVL SRVYELRNEI YIFLVEKQSH LANIFEDDIW VTKLAYLSDI FGILNELSLK
361MQGKNNDIFQ YLEHILGFQK TLLLWQARLK SNRPSYYMFP TLLQHIEENI INEDCLKEIK
421LEILLHLTSL SQTFNYYFPE EKFESLKENI WMKDPFAFQN PESIIELNLE PEEENELLQL
481SSSFTLKNYY KILSLSAFWI KIKDDFPLLS RKSILLLLPF TTTYLCELGF SILTRLKTKK
541RNRLNSAPDM RVALSSCVPD WKELMNRQAH PSH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

573 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.