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FAM196A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FAM196A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FAM196A antibodies →
$725.00each
Catalog Number: LS-G120386-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM196A (NM_001039762), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
FAM196A | Protein FAM196A | C10orf141
Origin Species
Human
NCBI GenBank
NM_001039762
SwissProt
Q6ZSG2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
52.7 kda
Protein Type
Lysate
Amino Acid Sequence
1MVSKDTGKCI LTTSESEVEP AACLALEMKY ALDPNRQIKK RNKALQVRFK DICEAQNEQR
61DTQLSSGQLG EKREAKPVSC RAAYRKYMTV PARRSIPNVT KSTGVQTSPD LKKCYQTFPL
121DRKKGNLKSL PAADPFKSQN NGFLTDAKEK NEAGPMEEAR PCGAGRVHKT TALVFHSNQH
181MNTVDQPLGV NCTEPCKSPE PLSYGEAALQ NSTRPPSEEP DYQLLGRAKQ DRGRPNSEEP
241APPALRRVFK TEVATVYAPA LSARAPEPGL SDSAAASQWS LCPADDERRR ATHLNGLQAP
301SETALACSPP MQCLSPECSE QPSQTHTPPG LGNQPSPTAV AAGEECQRIV PHTEVVDLKA
361QLQMMENLIS SSQETIKVLL GVIQELEKGE AHREGLSYRT GQDTANCDTC RNSACIIYSV
421ELDFKQQEDK LQPVLRKLHP IEETQVIPSP YSQETYSSTP KQKSKTESKK HGRWKLWFL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

479 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.