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FAM189A1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FAM189A1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FAM189A1 antibodies →
$504.00each
Catalog Number: LS-G127088-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM189A1 (NM_015307), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_015307

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
56.4 kda
Protein Type
Lysate
Amino Acid Sequence
1MPPAGGPRAP RPAALPRSLS RLRECPGRSR IVLALGATQM ALGCLIVAVS FAALALTTSA
61RVRHSCPFWA GFSVLLSGLI GVVSWKRPLS LVITFFMLLS AVCVMLNLAG SILSCQNAQL
121VNSLEGCQLI KFDSVEVCVC CELQHQSSGC SNLGETLKLN PLQENCNAVR LTLKDLLFSV
181CALNVLSTIV CALATAMCCM QMVSSDVLQM FLPQRSHPAN PTCVTPHGTV LHQTLDFDEF
241IPPLPPPPYY PPEYTCTPST EAQRGLHLDF APSPFGTLYD VAINSPGLLY PAELPPPYEA
301VVGQPPASQV TSIGQQVAES SSGDPNTSAG FSTPVPADST SLLVSEGTAT PGSSPSPDGP
361VGAPAPSEPA LPPGHVSPED PGMGSQVQPG PGRVSRSTSD PTLCTSSMAG DASSHRPSCS
421QDLEAGLSEA VPGSASMSRS ATAACRAQLS PAGDPDTWKT DQRPTPEPFP ATSKERPRSL
481VDSKAYADAR VLVAKFLEHS HCALPTEAQH MVGAMRLAVT NEERLEEEAV FGADVLDQV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

539 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.