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FAM188B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FAM188B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FAM188B antibodies →
$504.00each
Catalog Number: LS-G108975-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM188B (NM_032222), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
84.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDSLFVEEVA ASLVREFLSR KGLKKTCVTM DQERPRSDLS INNRNDLRKV LHLEFLYKEN
61KAKENPLKTS LELITRYFLD HFGNTANNFT QDTPIPALSV PKKNNKVPSR CSETTLVNIY
121DLSDEDAGWR TSLSETSKAR HDNLDGDVLG NFVSSKRPPH KSKPMQTVPG ETPVLTSAWE
181KIDKLHSEPS LDVKRMGENS RPKSGLIVRG MMSGPIASSP QDSFHRHYLR RSSPSSSSTQ
241PQEESRKVPE LFVCTQQDIL ASSNSSPSRT SLGQLSELTV ERQKTTASSP PHLPSKRLPP
301WDRARPRDPS EDTPAVDGST DTDRMPLKLY LPGGNSRMTQ ERLERAFKRQ GSQPAPVRKN
361QLLPSDKVDG ELGALRLEDV EDELIREEVI LSPVPSVLKL QTASKPIDLS VAKEIKTLLF
421GSSFCCFNEE WKLQSFSFSN TASLKYGIVQ NKGGPCGVLA AVQGCVLQKL LFEGDSKADC
481AQGLQPSDAH RTRCLVLALA DIVWRAGGRE RAVVALASRT QQFSPTGKYK ADGVLETLTL
541HSLTCYEDLV TFLQQSIHQF EVGPYGCILL TLSAILSRST ELIRQDFDVP TSHLIGAHGY
601CTQELVNLLL TGKAVSNVFN DVVELDSGDG NITLLRGIAA RSDIGFLSLF EHYNMCQVGC
661FLKTPRFPIW VVCSESHFSI LFSLQPGLLR DWRTERLFDL YYYDGLANQQ EQIRLTIDTT
721QTISEDTDND LVPPLELCIR TKWKGASVNW NGSDPIL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

757 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.