Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FAM188A / C10orf97 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G109638-100
Catalog Number: LS-G109638
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C10orf97(FAM188A) (NM_024948), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C10orf97 | CARD-containing protein | FAM188A | MST126 | MSTP126 | My042 | RP11-394I23.1 | Protein CARP | Protein FAM188A | CARP | DERP5
- Origin Species
- Human
- NCBI GenBank
- NM_024948
- SwissProt
- Q9H8M7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 49.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSELTKELME LVWGTKSSPG LSDTIFCRWT QGFVFSESEG SALEQFEGGP CAVIAPVQAF61LLKKLLFSSE KSSWRDCSEE EQKELLCHTL CDILESACCD HSGSYCLVSW LRGKTTEETA121SISGSPAESS CQVEHSSALA VEELGFERFH ALIQKRSFRS LPELKDAVLD QYSMWGNKFG181VLLFLYSVLL TKGIENIKNE IEDASEPLID PVYGHGSQSL INLLLTGHAV SNVWDGDREC241SGMKLLGIHE QAAVGFLTLM EALRYCKVGS YLKSPKFPIW IVGSETHLTV FFAKDMALVA301PEAPSEQARR VFQTYDPEDN GFIPDSLLED VMKALDLVSD PEYINLMKNK LDPEGLGIIL361LGPFLQEFFP DQGSSGPESF TVYHYNGLKQ SNYNEKVMYV EGTAVVMGFE DPMLQTDDTP421IKRCLQTKWP YIELLWTTDR SPSLNTRTRPLEQKLISEEDLAANDILDYKDDDDKV
445 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.