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FAM186B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FAM186B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FAM186B antibodies →
$504.00each
Catalog Number: LS-G109017-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM186B (NM_032130), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C12orf25 | FAM186B | Protein FAM186B | DKFZP434J0113
Origin Species
Human
NCBI GenBank
NM_032130
SwissProt
Q8IYM0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
103.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEKDDPPQLV TPTSVKAIIL RIEAAQLTRA QEDISTQLSD ILDNVNCVIN RFQEELGYDL
61KENAKSQQRD PKGKKRFILL EKIASFSKDA MMKEKHLYDI LRWLGDWGDT LTYEIGPRKS
121EEEAAALDEW IEVTEKVLPL SLIATKRGIE SLTALCSTLI EGQKKRSQVS KRTFWQGWQG
181RSPQTSPSHP QPLSPEQMLQ DQHTMNTKAS EVTSMLQELL DSTMFSKGEV RAIRYMATVV
241ENLNKALILQ HKENRSLETK YRHLQMQATK ELSSQRLHFQ QFMEVLESRR DALLKQVEIL
301GGRYHDLLLM KQALEFQLKK AQNATGQAED LAEVSVDSPG PSERETLPRK ETVMEESQQE
361PMKEEQLFSP LPPSPMAMIR DSGAIAAGHQ PLSTMTMRSR VADVFGSKDT ESLEPVLLPL
421VDRRFPKKWE RPVAESLGHK DKDQEDYFQK GGLQIKFHCS KQLSLESSRQ VTSESQEEPW
481EEEFGREMRR QLWLEEEEMW QQRQKKWALL EQEHQEKLRQ WNLEDLAREQ QRRWVQLEKE
541QESPRREPEQ LGEDVERRIF TPTSRWRDLE KAELSLVPAP SRTQSAHQSR RPHLPMSPST
601QQPALGKQRP MSSVEFTYRP RTRRVPTKPK KSASFPVTGT SIRRLTWPSL QISPANIKKK
661VYHMDMEAQR KNLQLLSEES ELRLPHYLRS KALELTTTTM ELGALRLQYL CHKYIFYRRL
721QSLRQEAINH VQIMKETEAS YKAQNLYIFL ENIDRLQSLR LQAWTDKQKG LEEKHRECLS
781SMVTMFPKLQ LEWNVHLNIP EVTSPKPKKC KLPAASPRHI RPSGPTYKQP FLSRHRACVP
841LQMARQQGKQ MEAVWKTEVA SSSYAIEKKT PASLPRDQLR GHPDIPRLLT LDV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

893 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.