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FAM184A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

FAM184A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FAM184A antibodies →
$504.00each
Catalog Number: LS-G109905-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM184A (NM_024581), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C6orf60 | FAM184A | Protein FAM184A
Origin Species
Human
NCBI GenBank
NM_024581
SwissProt
Q8NB25

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
132.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDYSQEMHLK LSKKIAQLTK VIYALNTKND EHESAIQALK DAHEEEIQQI LAETREKILQ
61YKSKVTEELD LRRKIQVLES SLEDHIKMKQ QALTEFEAYK HRVEDMQLCA EAQHVQRIVT
121MSREVEEIRR KFEEKLRSFG QLQVQFEKDK RLALEDLQAA HRQEIQELLK SQQDHSASVN
181KGQEKAEELH RMEVESLNKM LEELRLERKK LIEDYEGKLN KAQSFYEREL DTLKRSQLFT
241AESLQASKEK EADLRKEFQG QEAILRKTIG KLKTELQMVQ DEAGSLLDKC QKLQTALAIA
301ENNVQVLQKQ LDDAKEGEMA LLSKHKEVES ELAAARERLQ QQASDLVLKA SHIGMLQATQ
361MTQEVTIKDL ESEKSRVNER LSQLEEERAF LRSKTQSLDE EQKQQILELE KKVNEAKRTQ
421QEYYERELKN LQSRLEEEVT QLNEAHSKTL EELAWKHHMA IEAVHSNAIR DKKKLQMDLE
481EQHNKDKLNL EEDKNQLQQE LENLKEVLED KLNTANQEIG HLQDMVRKSE QGLGSAEGLI
541ASLQDSQERL QNELDLTKDS LKETKDALLN VEGELEQERQ QHEETIAAMK EEEKLKVDKM
601AHDLEIKWTE NLRQECSKLR EELRLQHEED KKSAMSQLLQ LKDREKNAAR DSWQKKVEDL
661LNQISLLKQN LEIQLSQSQT SLQQLQAQFT QERQRLTQEL EELEEQHQQR HKSLKEAHVL
721AFQTMEEEKE KEQRALENHL QQKHSAELQS LKDAHRESME GFRIEMEQEL QTLRFELEDE
781GKAMLASLRS ELNHQHAAAI DLLRHNHHQE LAAAKMELER SIDISRRQSK EHICRITDLQ
841EELRHREHHI SELDKEVQHL HENISALTKE LEFKGKEILR IRSESNQQIR LHEQDLNKRL
901EKELDVMTAD HLREKNIMRA DFNKTNELLK EINAALQVSL EEMEEKYLMR ESKPEDIQMI
961TELKAMLTER DQIIKKLIED NKFYQLELVN RETNFNKVFN SSPTVGVINP LAKQKKKNDK
1021SPTNRFVSVP NLSALESGGV GNGHPNRLDP IPNSPVHDIE FNSSKPLPQP VPPKGPKTFL
1081SPAQSEASPV ASPDPQRQEW FARYFTF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1107 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.