Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
FAM175B / KIAA0157 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G102032-100
Catalog Number: LS-G102032
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM175B (NM_032182), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Abraxas brother protein 1 | BRISC complex subunit Abro1 | Abraxas brother 1 | ABRO1 | Em:AC068896.4 | FAM175B | Protein FAM175B | KIAA0157
- Origin Species
- Human
- NCBI GenBank
- NM_032182
- SwissProt
- Q15018
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 46.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSYREQVLHK QLTRILGVPD LVFLLFSFIS TANNSTHALE YVLFRPNRRY NQRISLAIPN61LGNTSQQEYK VSSVPNTSQS YAKVIKEHGT DFFDKDGVMK DIRAIYQVYN ALQEKVQAVC121ADVEKSERVV ESCQAEVNKL RRQITQRKNE KEQERRLQQA VLSRQMPSES LDPAFSPRMP181SSGFAAEGRS TLGDAEASDP PPPYSDFHPN NQESTLSHSR MERSVFMPRP QAVGSSNYAS241TSAGLKYPGS GADLPPPQRA AGDSGEDSDD SDYENLIDPT EPSNSEYSHS KDSRPMAHPD301EDPRNTQTSQ ITRTRPLEQKLISEEDLAANDILDYKDDDDKV
311 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.